| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Slackia heliotrinireducens | TTGCCAGGGAGCTTGCAA | TATCGCGTGCTTGGCCAT | 59.80 | 59.81 | 194 | 130 |
100.00%
|
100.00%
|
| Primer 2 | Slackia heliotrinireducens | TTGCCAGGGAGCTTGCAA | TATCGCGTGCTTGGCCAT | 59.80 | 59.81 | 194 | 130 |
100.00%
|
100.00%
|
| Primer 3 | Slackia heliotrinireducens | CGTCGAATACGCCTACGAGG | GGAATATGGTCCTCGCGAGC | 60.38 | 60.67 | 239 | 121 |
100.00%
|
100.00%
|
| Primer 4 | Slackia heliotrinireducens | GTTCAACATGGCGACGCTG | TCGCGTCCTTGATCTGCTG | 60.15 | 60.15 | 254 | 110 |
100.00%
|
100.00%
|
| Primer 5 | Slackia heliotrinireducens | TTCAACATGGCGACGCTG | TCGCGTCCTTGATCTGCTG | 58.75 | 60.15 | 253 | 110 |
100.00%
|
100.00%
|
| Primer 6 | Slackia heliotrinireducens | TGGAGCCCTGCATCATGTG | TTTTCTGGCTGCGCTTGC | 60.08 | 59.66 | 219 | 108 |
100.00%
|
100.00%
|
| Primer 7 | Slackia heliotrinireducens | TGACGGCATGCTGTGGAA | AGCCGATGCACTTGTCGT | 59.57 | 59.66 | 209 | 97 |
100.00%
|
100.00%
|
| Primer 8 | Slackia heliotrinireducens | TGGCGTGCAAGTTCGAGA | AGCCGATGCACTTGTCGT | 59.58 | 59.66 | 237 | 97 |
100.00%
|
100.00%
|
| Primer 9 | Slackia heliotrinireducens | TCTCGTTTTCCGTCGGAGC | AGTCGCGTTGGGATTCTCC | 60.08 | 59.78 | 186 | 96 |
100.00%
|
100.00%
|
| Primer 10 | Slackia heliotrinireducens | TGAGAGCCACGACCTTTCC | ACGCCTCGACGTTGTTGT | 59.33 | 59.89 | 170 | 93 |
100.00%
|
100.00%
|